Description
This single 5-test UBeeO™ vial contains a pre-mixed, field-ready solution of synthetic brood pheromones used to assess hygienic behaviour (HB) in Apis mellifera colonies. It is identical in formulation to the vials supplied in the UBeeO™ Testing Kit and is ideal for:
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Beekeepers performing follow-up or mid-season testing
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Queen breeders evaluating additional colonies for selection
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Researchers requiring individual assay doses for field studies
Each vial contains enough solution to perform standardized tests on 5 colonies, using the UBeeO™ protocol and applicator system.
Scientific Application
The pheromone blend mimics chemical cues emitted by brood infected with Varroa destructor or bacterial pathogens. When applied to a 100-cell patch of capped worker brood, hygienic colonies will initiate uncapping and removal within 2 hours.
This method enables rapid, in-field screening of HB levels without damaging the brood, providing critical data for selection, breeding, or management decisions.
Use Instructions
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Use with the UBeeO™ applicator and test ring for precise and repeatable assays
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Apply to capped brood and return frame to hive
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After 2 hours, assess % of uncapped cells
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Colonies uncapping ≥60% are classified as high-hygiene and suitable for selection
Storage & Handling
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Shelf Life (Unopened): ~3 years if stored at –18°C (freezer)
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Post-Opening: For best results, use the vial within 24 hours of opening
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Storage:
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Long-term: Freezer (–18°C)
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Short-term (1–2 weeks): Refrigerator (4°C), though reduced efficacy may occur
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Proper cold storage is essential to preserve volatile activity of pheromone components.
Technical Specifications
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Volume: 1 x 5-test vial (sufficient for 5 colonies)
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Formulation: Synthetic brood pheromone analogues
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Compatibility: Use with UBeeO™ applicator system
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Shipping: Shipped chilled or with insulation; storage upon arrival recommended
Ideal For
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Queen breeders expanding HB screening across mating yards
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Beekeepers conducting re-tests or supplemental assays
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Researchers requiring standardized stimuli for controlled experiments